Robert D. Cardiff, MD, PhD


cardiff-robertDistinguished Professor Emeritus

SOM: Pathology and Laboratory Medicine
Center for Genomic Pathology
University of California, Davis
County Rd. 98 & Hutchison Dr.
Davis, CA 95616
Phone: (530) 752-0332
Fax: (530) 752-7914
rdcardiff@ucdavis.edu

Research Interests: Comparative Pathology, Comparative Oncology, Informatics

Dr. Cardiff is an academic surgical pathologist who received his bachelor’s degree (entomology) and Ph.D. (zoology) from UC Berkeley and his MD degree from UCSF. His pathology training was at Univ.Oregon Medical School, Portland. He has been at University of California, Davis since 1971 where he is a Distinguished Professor and was the Chair of the Department of Pathology from 1990 to 1996, Founding Director of the UCD Center for Medical Informatics and UCD Center for Comparative Medicine’s Mutant Mouse Pathology Facility (MMPF). His research interests have focused on experimental pathology of mouse tumorigenesis. As director of the MMPF, he has developed a digitized archive of samples from over 30,000 mutant mice from over 200 laboratories from 18 countries. Whole slide imaging has allowed him to work with scientists in Europe and throughout the world. This extensive collection serves as a basis for many of his current studies and provides him a unique niche in science as one of the few surgical pathologists in the world who combines a knowledge of R01 funded research, human pathology and mouse tumor pathology. He has headed the Pathology Core for the NCI Mouse Models of Human Cancers Consortium and has been a Lead Pathologist for the UC-wide Athena Breast Cancer Research Program. He is the founder and Director of the Center for Genomic Pathology which is devoted to education in comparative pathology. His current research emphasizes mouse models of mammary cancer. Most recently, he has focused attention of the scientific community on the need for comparative pathologists with training necessary for evaluation of the pending plethora of genetically modified mice.

Mutant Mouse Pathology Laboratory 

Dr. Cardiff’s laboratory provides comprehensive histopathology services to over 200 investigators in over 200 laboratories in 15 countries.  The archive includes blocks and slides from over 18,000 cases representing 200 types of genetically engineered mice, and is a unique research and training resource that is unparalleled in the world. The comparative studies have provided unique insights into the molecular and functional genomics of cancer.  The comparison of structure and function has led to the recognition that genetically engineered mice have unique forms of cancer.  Each gene produces a characteristic, and frequently unique, type of cancer.  Many of these cancers closely resemble human cancers.  Cancers of the breast, white blood cells, skin, lung, salivary gland, prostate, bone, liver, and mesenchyme have been studied. Studies done in this facility with collaborators have resulted in over 70 papers since 1990.

Transgenic Mouse Tumor Biology 

Studies based on murine models have involved molecular biology, transplantation biology, tumor biology, immunohistochemistry, light microscopy, in-situ hybridization, special histochemistry, image analysis and morphometrics.  Recent studies have concentrated on angiogenesis and transgene expression. Current studies emphasize the early phases of tumor development using tissue transplantation. The research team has developed the first transgenic mouse model of precancer of the human breast.

Engelberg JA, Giberson RT, Young LJ, Hubbard NE, Cardiff RD. The use of mouse models of breast cancer and quantitative image analysis to evaluate hormone receptor antigenicity after microwave-assisted formalin fixation. J Histochem Cytochem. 2014 May;62(5):319-34. doi:  0.1369/0022155414529250. Epub 2014 Mar 28. PubMed PMID: 24682322; PubMed Central PMCID:  PMC4005368.

Cardiff RD, Borowsky AD. At last: classification of human mammary cells elucidates breast cancer origins. J Clin Invest. 2014 Feb 3;124(2):478-80. doi: 10.1172/JCI73910. Epub 2014 Jan 27. PubMed PMID: 24463442; PubMed Central PMCID:  PMC3904633.

Paoli EE, Ingham ES, Zhang H, Mahakian LM, Fite BZ, Gagnon MK, Tam S, Kheirolomoom A, Cardiff RD, Ferrara KW. Accumulation, internalization and therapeutic efficacy of neuropilin-1-targeted liposomes. J Control Release2014 Mar 28;178:108-17.PMCID:4079909.PMID.24434424.

Hollern DP, Honeysett J, Cardiff RD, Andrechek ER. The E2F transcription factors regulate tumor development and metastasis in a mouse model of metastatic breast cancer. Mol Cell Biol2014 Jun 16.PMID.24934442.

Feigin ME, Akshinthala SD, Araki K, Rosenberg AZ, Muthuswamy LB, Martin B, Lehmann BD, Berman HK, Pietenpol JA, Cardiff RD, Muthuswamy SK. Mislocalization of the cell polarity protein scribble promotes mammary tumorigenesis and is associated with basal breast cancer. Cancer Res2014 Jun 1;74(11):3180-94.PMCID:4096808.PMID.24662921.

Cardiff RD, Miller CH, Munn RJ, Galvez JJ. Structured reporting in anatomic pathology for coclinical trials: the caELMIR model. Cold Spring Harb Protoc2014 Jan;2014(1):32-43.PMID.24173313.

Cardiff RD, Miller CH, Munn RJ. Limited mouse necropsy. Cold Spring Harb Protoc 2014 May; 2014(5).PMID.24786500.

Cardiff RD, Miller CH, Munn RJ. Mouse tissue fixation. Cold Spring Harb Protoc 2014 May; 2014(5).PMID.24786501.

Cardiff RD, Miller CH, Munn RJ. Manual hematoxylin and eosin staining of mouse tissue sections. Cold Spring Harb Protoc2014 Jun; 2014(6):655-8.PMID.24890205.

Cardiff RD, Miller CH, Munn RJ. Manual immunohistochemistry staining of mouse tissues using the avidin-biotin complex (ABC) technique. Cold Spring Harb Protoc2014 Jun; 2014(6):659-62.PMID.24890206.

Cardiff RD, Miller CH, Munn RJ. Analysis of mouse model pathology: a primer for studying the anatomic pathology of genetically engineered mice. Cold Spring Harb Protoc2014 Jun;2014(6):561-80.PMID.24890215.

Meyer DS, Koren S, Leroy C, Brinkhaus H, Muller U, Klebba I, Muller M, Cardiff RD, Bentires-Alj M. Expression of PIK3CA mutant E545K in the mammary gland induces heterogeneous tumors but is less potent than mutant H1047R. Oncogenesis2013;2:e74.PMID.24080956.

Kurpios NA, Girgis-Gabardo A, Hallett RM, Rogers S, Gludish DW, Kockeritz L, Woodgett J, Cardiff R, Hassell JA. Single unpurified breast tumor-initiating cells from multiple mouse models efficiently elicit tumors in immune-competent hosts. PLoS One2013;8(3):e58151.PMCID:3608640.PMID.23555570.

Haricharan S, Hein SM, Dong J, Toneff MJ, Aina OH, Rao PH, Cardiff RD, Li Y. Contribution of an alveolar cell of origin to the high-grade malignant phenotype of pregnancy-associated breast cancer. Oncogene2013 Dec 9.PMID.24317513.

Fujita M, Ieguchi K, Cedano-Prieto DM, Fong A, Wilkerson C, Chen JQ, Wu M, Lo SH, Cheung AT, Wilson MD, Cardiff RD, Borowsky AD, Takada YK, Takada Y. An Integrin Binding-defective Mutant of Insulin-like Growth Factor-1 (R36E/R37E IGF1) Acts as a Dominant-negative Antagonist of the IGF1 Receptor (IGF1R) and Suppresses Tumorigenesis but Still Binds to IGF1R. J Biol Chem2013 Jul 5;288(27):19593-603.PMID.23696648.

Cardiff RD, Miller CH, Munn RJ, Galvez JJ. Structured reporting in anatomic pathology for coclinical trials: the caELMIR model. Cold Spring Harb Protoc2014 Jan;2014(1):32-43.PMID.24173313.

Cardiff RD, Miller CH, Munn RJ. Limited mouse necropsy. Cold Spring Harb Protoc2014 May;2014(5).PMID.24786500.

Cardiff RD, Miller CH, Munn RJ. Mouse tissue fixation. Cold Spring Harb Protoc2014 May;2014(5).PMID.24786501.

Cardiff RD, Miller CH, Munn RJ. Manual hematoxylin and eosin staining of mouse tissue sections. Cold Spring Harb Protoc2014 Jun;2014(6):655-8.PMID.24890205.

Cardiff RD, Miller CH, Munn RJ. Manual immunohistochemistry staining of mouse tissues using the avidin-biotin complex (ABC) technique. Cold Spring Harb Protoc2014 Jun;2014(6):659-62.PMID.24890206.

Cardiff RD, Miller CH, Munn RJ. Analysis of mouse model pathology: a primer for studying the anatomic pathology of genetically engineered mice. Cold Spring Harb Protoc2014 Jun;2014(6):561-80.PMID.24890215.

Hubbard, Neil

Project Scientist

Sugandha, Herlina

SRA II

Walls, Judith

SRA III